ESR projects

ESR 1

Host institution POLITECNICO DI TORINO — Torino — Italy (POLITO)

Location Torino — Italy

Project Title Materiomics analysis of metallic and ceramic materials for musculoskeletal regeneration

Objectives and project description

The project objective is to investigate the links between physico-chemical properties of metallic (titanium and its alloys and ceramic (bioactive glasses and glass-ceramics) materials, including chemistry (chemical composition, surface functionalization), surface morphology (topography, roughness) and hierarchical porosity (at the macro-, meso-nanoscale), and cells (pro- and eukaryotic) and tissues functionality. Optimization of the materials for specific cells and tissues responses is the core activity of the project.

Supervisors and contact person Prof. Enrica Vernè 

ESR 2

Host institution RIGA TECHNICAL UNIVERSITY — Riga — Latvia (RTU)

Location Riga — Latvia

Project Title Smart personalized degradable biomaterials for bone tissue regeneration

Objectives and project description

The project objective is to develop personalized drug delivery systems to incorporate in biomaterials for bone tissue regeneration, including two levels of personalization:

1) Biomaterial composition; (faster or slower degrading, depending on patient and application needs);

2) Drug delivery personalization; including what related to kinetics and doses.

Supervisors and contact persons Prof. Janis Locs, Assoc. Prof Dagnija Loca

ESR 3

Host institution TAMPEREEN KORKEAKOULUSAATIO SR — Tampere — Finland (TAU)

Location Tampere — Finland

Project Title The role of proteins dynamics in biomaterials/ bone biosystem

Objectives and project description

The research project objective is to identify the physical-chemistry properties of the surface of biomaterials (metals and ceramics) influence on adsorption and kinetics of specific proteins promoter for musculoskeletal tissue regeneration.

 This project analyses the impact of surface chemistry, charge and topography on model protein adsorptions and conformation. Impact of protein adsorption at the surface of the biomaterials on the stem cells fate will be also assessed. 

Supervisors and contact person Assoc. Prof. Jonathan Massera

ESR 4

Host institution FACULTY OF TECHNOLOGY AND METALLURGY — University of Belgrade — Belgrade — Serbia (UOB)

Location Belgrade — Serbia

Project Title Biomimetic bioreactor systems as physiologically relevant environments

Objectives and project description

The project objective is to develop and utilize in vitro biomimetic bioreactor systems as physiologically relevant environments for in vitro tissue regeneration as well as for evaluation of novel biomaterials and prediction of the biomaterial function upon implantation. The core activity of the project consists of assessment and optimization of bioceramic and composite biomaterials for bone/osteochondral tissue regeneration in the biomimetic bioreactor systems.

Supervisors and contact person Prof. Dr. Bojana Obradovic 

ESR 5

Host institution ISTITUTO ORTOPEDICO RIZZOLI — Bologna — Italy (IOR)

Location Bologna — Italy

Project Title Smart personalized degradable biomaterials for bone tissue regeneration

Objectives and project description

The project objectives are:

1) To deepen knowledge of the clinical problems in regeneration of bone and cartilage and of orthopedic reconstructive surgery fields from animal retrospective studies, collecting data on the regenerative/reparative steps and mechanisms reproducible in vitro

2) To develop 3-D advanced in vitro models simulating the cellular and tissue microenvironment during the onset and progression of particular pathologies of the musculoskeletal system, such as osteoarthritis, osteolysis and alterations of bone metabolism including osteoporosis;

3) To develop and assess 3-D advanced in vitro models, consisting of human cells and tissues, for “mechanistic” studies necessary for the understanding of mechanisms of action, toxicity and dose-effect of therapeutic innovative treatments and, at the same time, for reducing the number of animals used for experimental purposes.

Supervisors and contact person Dr. Milena Fini, MD

ESR 6

Host institution AO RESEARCH INSTITUTE — Davos — Switzerland (ARI)

Location Davos — Switzerland

Project Title Design of 3D in vitro vascularized organoid model

Objectives and project description

The project objectives are to:

1) Develop in vitro 3D organoid models able to study patient specific tissue behaviour for potential novel treatment and/or implementation in regulatory process;

2) Obtain in vitro safety/efficacy data on the “specific treatment” using the developed 3D organoid models and compare with in vivo data;

3) Learning bioprinting / acoustic patterning process for the generation of 3D organoid models;

4) Develop, print and characterization bioinks for 3D organoid models;

5) Produce perfusable tubular, vascular networks within the 3D organoid models;

6) Establish 3D organoid models’ functionality in term of ability to read-out “drugs response”.

Supervisors and contact persons Prof. Mauro Alini,  Dr. Tiziano Serra

ESR 7

Host institution INSTITUTO NACIONAL DE ENGENHARIA BIOMEDICA — Porto — Portugal (INEB)

Location Porto — Portugal

Project Title 3D microfluidic based models for testing innervation of engineered microtissues for medical applications

Objectives and project description

The project objective is to development of 3-D microfluidic based models to address the ability of cartilage engineered microtissues for joints medical applications to promote or avoid peripheral tissue innervation. ESR7 will acquire skills in: Fabrication of compartmentalized microfluidic devices (CMV), in vitro co-cultures in CMV, 3D neuronal/non-neuronal co-cultures, 3D neuronal/non-neuronal co-cultures in CMV, readout of cellular interactions in CMV.

Supervisors and contact persons:  Prof. Meriem Lamghari,  Dr. Estrela Neto, Dr. Ana Paula Filipe

ESR 8

Host institution INSTITUTO NACIONAL DE ENGENHARIA BIOMEDICA — Porto — Portugal (INEB)

Location Porto — Portugal

Project Title Innervation profile of cartilage engineered constructs under exposure to inflammatory insults

Objectives and project description

The project objective is to investigate the effect of the proinflammatory environment on the neurotrophic vs repulsive ability of the cartilage engineered constructs. ESR8 will acquire skills in Macrophages cultures and polarization, 3-D neuronal/non-neuronal cocultures in CMV, assessment of the innervation profile triggered by cartilage engineered constructs under stimulation of the proinflammatory vs anti-inflammatory macrophages secretomes, cell and molecular biology techniques.

Supervisors and contact persons: Prof. Meriem Lamghari, Dr. Estrela Neto, Dr Ana Paula Filipe

ESR 9

Host institution UNIVERSITA’ DEL PIEMONTE ORIENTALE – Alessandria, Novara, Vercelli — Italy (UPO)

Location Novara — Italy

Project Title Extracellular microvesicles and exosomes (EVs) as biomarkers/mediators of stem cells differentiation, wound healing and tissue regeneration around materials

Objectives and project description

The project objectives are to:

1) Characterize EV and exosomes in emblematic patient groups as biomarkers predictive of tissue healing and regeneration (proteomics, transcriptomics);

2) Identify the angiogenetic and neurogenetic role of EVs and exosome around materials;

3) Promote the release of angiogenetic and neurogenetic factors from EVs and exosome by contact with optimized biomaterials.

Supervisors and contact person Prof. Annalisa Chiocchetti

ESR 10

Host institution UNIVERSITA’ DEL PIEMONTE ORIENTALE – Alessandria, Novara, Vercelli — Italy (UPO)

Location Novara — Italy

Project Title Understanding the function of immune-system cells and cellular stress adaptation during healing

Objectives and project description

The project objective is to develop protocols to evaluate immune and cellular stress responses, stress adaptation and cell death during healing and tissue regeneration in presence with biomaterials.

Supervisors and contact person Prof. Marco Corazzari 

ESR 11

Host institution AALTO KORKEAKOULUSAATIO SR — Espoo — Finland (AALTO)

Location Espoo — Finland

Project Title Evaluation of in vitro degradation and associated risks of novel biomaterials for implants

Objectives and project description

The project objective is to assessment in vitro of the risks of novel biomaterials solutions under pseudo-static and dynamic conditions vs. conventional controls.

Supervisors and contact person Prof. Michael Gasik 

ESR 12

Host institution ENGINSOFT SpA — Padova — Italy (ES)

Location Padova — Italy

Project Title Multi-objective design and optimization (MODAO) of the data from in vitro and in vivo tests data

Objectives and project description

The project objective is to use advanced data processing and data mining methods to discover “hidden links” between the experimental and clinical variables capable to generate greater level of evidence and to generate predictive models leading to the DSS.

Supervisors and contact person Dr. Manolo Venturin 

ESR 13

Host institution NATIONAL UNIVERSITY OF IRELAND — Galway — Ireland (NUI)

Location Galway — Ireland

Project Title Development of a glycol-functionalised personalised hydrogel for interfacial tissue repair

Objectives and project description

The project objectives are to:

1) Validate common specific glycosignature profiles on rat, sheep and human NP and AF cells and tissue;

2) Functionalize a Type II collagen/hyaluronan hydrogel with glycosaminoglycans (GAGs) and/or proteoglycans (PG) motifs to maintain the phenotype and viability of patient derived bone marrow derived stem cells;

3) Evaluate the efficacy of the collagen/hyaluronan reservoir system to maintain the microenvironment, enhance GAG production and slow down disc degeneration in an organ culture model;

4) Evaluate the efficacy of the cell seeded glyco-functionalised Type II collagen/hyaluronan hydrogel to recapitulate the biosynthesis of functional extracellular matrix (ECM) in vivo.

Supervisors and contact person Prof. Abhay Pandit ,